Kim, Jee Young; Sung, Gun Yong; Park, Min published an article in 2020, the title of the article was Efficient portable urea biosensor based on urease immobilized membrane for monitoring of physiological fluids.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:
Herein, a portable urea biosensor was developed for the real-time monitoring of the flow of physiol. fluids; this was achieved by using disuccinimidyl cross-linker-based urease immobilization. Urease was immobilized on a porous polytetrafluoroethylene (PTFE) solid support using different disuccinimidyl cross-linkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5). A urease activity test revealed that DSS exhibited the highest urease immobilizing efficiency, whereas FT-IR anal. confirmed that urease was immobilized on the PTFE membrane via DSS crosslinking. The membrane was inserted in a polydimethylsiloxane (PDMS) fluidic chamber that generated an electrochem. signal in the presence of a flowing fluid containing urea. Urea samples were allowed to flow into the urea biosensor (1.0 mL/min) and the signal was measured using chronoamperometry. The sensitivity of the DSS urea biosensor was the highest of all the trialed biosensors and was found to be superior to the more commonly used GA cross-linker. To simulate real-time monitoring in a human patient, flowing urea-spiked human serum was measured and the effective urease immobilization of the DSS urea biosensor was confirmed. The repeatability and interference of the urea biosensor were suitable for monitoring urea concentration typically found in human patients. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate
The Article related to body fluid urease urea biosensor, disuccinimidyl cross-linker, flow system, real-time monitoring, urea biosensor, urease immobilization, Biochemical Methods: Biological and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate
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