On April 5, 2016, Killinger, Bryan A.; Moszczynska, Anna published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Characterization of α-Synuclein Multimer Stoichiometry in Complex Biological Samples by Electrophoresis. And the article contained the following:
The aberrant aggregation of α-synuclein in the brain is a hallmark of Parkinson’s disease (PD). In vivo soluble α-synuclein occurs as a monomer and several multimers, the latter of which may be important for the biol. function of α-synuclein. Currently, there is a lack of reproducible methods to compare α-synuclein multimer abundance between complex biol. samples. Here we developed a method, termed “multimer-PAGE,” that combines in-gel chem. crosslinking with several common electrophoretic techniques to measure the stoichiometry of soluble α-synuclein multimers in brain tissue lysates. Results show that soluble α-synuclein from the rat brain exists as several high mol. weight species of approx. 56 kDa (αS56), 80 kDa (αS80), and 100 kDa (αS100) that comigrate with endogenous lipids, detergents, and/or micelles during blue native gel electrophoresis (BN-PAGE). Co-extraction of endogenous lipids with α-synuclein was essential for the detection of soluble α-synuclein multimers. Homogenization of brain tissue in small buffer volumes (>50 mg tissue per 1 mL buffer) increased relative lipid extraction and subsequently resulted in abundant soluble multimer detection via multimer-PAGE. α-Synuclein multimers captured by directly crosslinking soluble lysates resembled those observed following multimer-PAGE. The ratio of multimer (αS80) to monomer (αS17) increased linearly with protein input into multimer-PAGE, suggesting to some extent, multimers were also formed during electrophoresis. Overall, soluble α-synuclein maintains lipid interactions following tissue disruption and readily forms multimers when this lipid-protein complex is preserved. Once the multimer-PAGE technique was validated, relative stoichiometric comparisons could be conducted simultaneously between 14 biol. samples. Multimer-PAGE provides a simple inexpensive biochem. technique to study the mol. factors influencing α-synuclein multimerization. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
The Article related to synuclein multimer assembly stoichiometry determination electrophoresis, Biochemical Methods: Electrical and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
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