On October 15, 1980, Johansen, Jack Taaning; Widmer, Fred published a patent.SDS of cas: 53838-27-0 The title of the patent was Enzymic production of peptides. And the patent contained the following:
Peptides are produced by reacting amino acid esters, peptide esters, depsipeptides, etc., with an amino acid in the presence of carboxypeptidase in an aqueous solution with a pH of 5-10.5. Thus, a valine [72-18-4]-KCl-EDTA solution, pH 9.8, was mixed with a Bz-Ala-OMe [7244-67-9] solution and the reaction carried out in a pH-stat at 35° with the pH maintained by the addition of NaOH. The reaction was initiated by adding carboxypeptidase Y [9046-67-7]. It was stopped after sufficient time by the addition of HCl to lower the pH to 1.0. The reaction product was purified and isolated by high-pressure chromatog. giving a 40% yield of Bz-Ala-Val-OH [71448-06-1]. The experimental process involved the reaction of (S)-5-tert-Butyl 1-methyl 2-aminopentanedioate(cas: 53838-27-0).SDS of cas: 53838-27-0
The Article related to peptide production carboxypeptidase, amino acid peptide production enzyme, amide peptide production enzyme, Fermentations: Other and other aspects.SDS of cas: 53838-27-0
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics