ãSynthesis and mass spectra of rearrangement bio-signature metabolites of anaerobic alkane degradation via fumarate additionã?was published in Analytical Biochemistry in 2020. These research results belong to Chen, Jing; Zhou, Lei; Liu, Yi-Fan; Hou, Zhao-Wei; Li, Wei; Mbadinga, Serge Maurice; Zhou, Jing; Yang, Tao; Liu, Jin-Feng; Yang, Shi-Zhong; Wu, Xiao-Lin; Gu, Ji-Dong; Mu, Bo-Zhong. Recommanded Product: Diethyl 2-methylmalonate The article mentions the following:
Metabolite profiling in anaerobic alkane biodegradation plays an important role in revealing activation mechanisms. Apart from alkylsuccinates, which are considered to be the usual biomarkers via fumarate addition, the downstream metabolites of C-skeleton rearrangement can also be regarded as biomarkers. However, it is difficult to detect intermediate metabolites in both environmental samples and enrichment cultures, resulting in lacking direct evidence to prove the occurrence of fumarate addition pathway. A synthetic method of rearrangement metabolites was established. Four compounds, namely, propylmalonic acid, 2-(2-methylbutyl)malonic acid, 2-(2-methylpentyl)malonic acid and 2-(2-methyloctyl)malonic acid, were synthesized and determined by four derivatization approaches. Besides, their mass spectra were obtained. Four characteristic ions were observed at m/z 133 + 14n, 160 + 28n, 173 + 28n and [M – (45 + 14n)]+ (n = 0 and 2 for Et and Bu esters, resp.). For Me esterification, mass spectral features were m/z 132, 145 and [M – 31]+, while for silylation, fragments were m/z 73, 147, 217, 248, 261 and [M – 15]+. These data provide basis on identification of potential rearrangement metabolites in anaerobic alkane biodegradation via fumarate additionDiethyl 2-methylmalonate(cas: 609-08-5Recommanded Product: Diethyl 2-methylmalonate) was used in this study.
Diethyl 2-methylmalonate(cas: 609-08-5) belongs to aliphatic hydrocarbons. Aliphatic hydrocarbons belong to the most abundant fraction in crude oil. Aliphatics molecules are linear or branched open-chain structures such as n-alkanes, isoalkanes, cycloalkanes (naphthenes), terpenes and steranes.Recommanded Product: Diethyl 2-methylmalonate
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