On July 31, 2021, Budde, Dana; Albano, Gian-Luca; Noll, Thomas; Jurkiewicz, Elke published an article.COA of Formula: C33H54O6 The title of the article was Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation. And the article contained the following:
The presence of leachables in biopharmaceutical processes using single-use technologies (SUT) is well known. For the detection and quantification of the latter, extractable studies of SUT are very common nowadays. Although a mixture of compounds is regularly found in extractable studies, research has only been carried out regarding the effect of individual compounds on cell culture and the cumulative effect of a mix of leachables has not been investigated yet. In this study, a set of leachable model compounds (LMCs) was chosen and the effect of the LMCs on a Chinese hamster ovary DG44 cell line producing an IgG antibody was investigated concerning cell growth, cell cycle distribution and productivity. It was shown that even if worst-case concentrations were used, the LMCs solely impact cell growth. Addnl., interaction studies revealed that the inhibiting effect of the mix is lower than the expected cumulative effect. A strong antagonism between the antioxidant butylated hydroxytoluene and the plasticizer Tris(2-ethylhexyl)trimellitate was found using an isobologram anal. The experimental process involved the reaction of Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate(cas: 3319-31-1).COA of Formula: C33H54O6
The Article related to leachable model compound hamster ovary cell cultivation, antibody production, cytotoxicity, extractables and leachables, isobologram analysis, single-use systems, Nonmammalian Biochemistry: Other and other aspects.COA of Formula: C33H54O6
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