Antipchik, Mariia; Polyakov, Dmitry; Sinitsyna, Ekaterina; Dzhuzha, Apollinariia; Shavlovsky, Mikhail; Korzhikova-Vlakh, Evgenia; Tennikova, Tatiana published an article in 2020, the title of the article was Towards the development of a 3-D biochip for the detection of hepatitis C virus.Synthetic Route of 2358-84-1 And the article contains the following content:
The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The anal. platforms of suggested devices were based on macroporous poly(glycidyl methacrylate-co-di(ethylene glycol) dimethacrylate) monolithic material. The biochips were obtained by the covalent immobilization of specific probes spotted onto the surface of macroporous monolithic platforms. Using the developed biochips, different variants of bioassay were investigated. This study was carried out using hepatitis C virus-mimetic particles (VMPs) representing polymer nanoparticles with a size close to HCV and bearing surface virus antigen (E2 protein). At the first step, the main parameters of bioassay were optimized. Addnl., the dissociation constants were calculated for the pairs “ligand-receptor” and “antigen-antibody” formed at the surface of biochips. As a result of this study, the anal. of VMPs in model buffer solution and human blood plasma was carried out in a format of direct and “sandwich” approaches. It was found that bioassay efficacy appeared to be similar for both the model medium and real biol. fluid. Finally, limit of detection (LOD), limit of quantification (LOQ), spot-to-spot and biochip-to-biochip reproducibility for the developed systems were evaluated. The experimental process involved the reaction of Oxybis(ethane-2,1-diyl) bis(2-methylacrylate)(cas: 2358-84-1).Synthetic Route of 2358-84-1
The Article related to biochip 3d gma degdma antigen antibody hepatitis c virus, biochips, hepatitis c, macroporous monolithic polymer layers, microarray, virus-mimetic particles, Biochemical Methods: Biological and other aspects.Synthetic Route of 2358-84-1
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