Xia, Yijun; Wang, Youbin; Xiao, Yingjie; Shan, Mengjie; Hao, Yan; Zhang, Lingyun published an article in 2022, the title of the article was Identification of a diagnostic signature and immune cell infiltration characteristics in keloids.Electric Literature of 2358-84-1 And the article contains the following content:
Keloid disorder is a recurrent fibroproliferative cutaneous tumor. Due to the lack of early identification of keloid patients before the formation of keloids, it is impossible to carry out pre-traumatic intervention and prevention for these patients. This led us to identify and determine signatures with diagnostic significance for keloids. Public series of matrix files were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were calculated from expression profiling data, and the diagnostic signature was identified by constructing a protein-protein interaction (PPI) network. The diagnostic efficacy of the screened signature was assessed by employing receiver operating characteristic (ROC) curves. Furthermore, we calculated the proportion of different immune cells in the gene expression matrix microenvironment by the “ssGSEA” algorithm, and assessed the difference in immune cell abundance between keloids and control groups and the relationship between the signature and immune cell infiltration. Clin. keloid and normal skin tissues were collected, and the expression of the screened diagnostic signature was validated by RT-qPCR and immunohistochem. assay. By screening the key genes in PPI, TGM2 was recognized and validated as a diagnostic signature and the infiltrating abundance of 10 immune cells was significantly correlated with TGM2 expression. Gene ontol. enrichment anal. demonstrated that TGM2 and mols. interacting with it were mainly enriched in processes involving wound healing and collagen fiber organization. TGM2 correlated pos. with HIF-1A (R = 0.82, p-value = 1.4e-05), IL6 (R = 0.62, p-value = 0.0053), and FN1 (R = 0.66, p-value = 0.0019). Besides, TGM2 was significantly upregulated in clin. keloid samples compared to normal skin tissues. TGM2 may serve as an auxiliary diagnostic indicator for keloids. However, the role of TGM2 in keloids has not been adequately reported in the current literature, which may provide a new direction for mol. studies of keloids. The experimental process involved the reaction of Oxybis(ethane-2,1-diyl) bis(2-methylacrylate)(cas: 2358-84-1).Electric Literature of 2358-84-1
The Article related to immune cell infiltration protein interaction skin keloids diagnosis human, tgm2, diagnostic signature, immune cell infiltration, keloid, ssgsea, Mammalian Pathological Biochemistry: Skin Diseases and other aspects.Electric Literature of 2358-84-1
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