On March 31, 2011, Santos, Luiz Fernando Arruda; Eberlin, Marcos Nogueira; Gozzo, Fabio Cesar published an article.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was IRMPD and ECD fragmentation of intermolecular cross-linked peptides. And the article contained the following:
Despite the increasing number of studies using mass spectrometry for three dimensional analyses of proteins (MS3D), the identification of crosslinked peptides remains a bottleneck of the method. One of the main reasons for this is the lack of knowledge about the fragmentation of these species. Intermol. crosslinked peptides are considered the most informative species present in MS3D experiment, since different peptides are connected by a crosslinker, the peptides chain can be either from a single protein, providing information about protein folding, or from two different proteins in a complex, providing information about binding partners, complex topol. and interaction sites. These species tend to be large and highly charged in ESI, making comprehensive fragmentation by CID MS/MS problematic. On the other hand, these highly charged peptides are very suitable for dissociation using both IR multiphoton dissociation (IRMPD) and electron capture dissociation (ECD). Herein, we report the fragmentation study of intermol. crosslinked peptides using IRMPD and ECD. Using synthetic peptides and different com. crosslinkers, a series of intermol. crosslinked peptides were generate, and subsequently fragmented by IRMPD and ECD in a FT-ICR-MS instrument. Due to the high mass accuracy and resolution of the FT-ICR, the fragment ions could be attributed with high confidence. The peptides sequence coverage and fragmentation features obtained from IRMPD and ECD were compared for all charge states. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
The Article related to irmpd ecd fragmentation intermol cross linked peptide ms, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
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