Natrajan, Anand; Wen, David published an article in 2015, the title of the article was A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:
Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that were used in automated assays for clin. diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alk. peroxide. Conjugation of acridinium ester labels is normally done at the phenol. During the chemiluminescent reaction of these acridinium esters, the phenolic ester is cleaved and the light emitting acridone moiety is liberated from its conjugate partner. In the current study, the authors report the synthesis of three new acridinium esters with conjugation sites at the acridinium nitrogen and compare their properties with that of a conventional acridinium ester with a conjugation site at the phenol. The authors’ study is the first that provides a direct comparison of the emissive properties of acridinium dimethylphenyl esters (free labels and protein conjugates) with different conjugation sites, one where the light emitting acridone remains attached to its conjugate partner vs. conventional labeling which results in cleavage of the acridone from the conjugate. The authors’ results indicate that the conjugation at the acridinium nitrogen, which also alters how the acridinium ring and phenol are oriented with respect to the protein surface, has a minimal impact on emission kinetics and emission spectra. However, this mode of conjugation to three different proteins led to a significant increase in light yield which should be useful for improving the assay sensitivity. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
The Article related to chemiluminescent acridinium dimethylphenyl ester label conjugation site protein, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate
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