On May 1, 2016, Kowata, Keiko; Kojima, Naoshi; Komatsu, Yasuo published an article.Application of 79642-50-5 The title of the article was Development of a 3′-amino linker with high conjugation activity and its application to conveniently cross-link blunt ends of a duplex. And the article contained the following:
The 2-aminoethyl carbamate linker (ssH linker) exhibits high activity in modifying the 5′-termini of oligonucleotides; however, the ssH linker is not appropriate for 3′-terminal modification because it undergoes intramol. trans-acylation under heat-aqueous ammonia conditions. We developed an N-(2-aminoethyl)carbamate linker (revH linker), in which the carbamate is oriented in the reverse direction relative to that in 2-aminoethyl carbamate. The revH linker was tolerant to heat-alk. conditions and retained its high reactivity in conjugation with exogenous mols. The 3′-revH linker was efficiently linked with the 5′-ssH linker at the termini of complementary double strands with a bifunctional mol., producing a synthetic loop structure. An anti-microRNA oligonucleotide (AMO) was prepared from the chem. ligation of three-stranded 2′-O-Me RNAs, and the AMO with two alkyl loops exhibited high inhibition activity toward miRNA function. The revH linker is not only useful for 3′-terminal modification of oligonucleotides but also expands the utility range in combination with the 5′-ssH linker. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5
The Article related to microrna dna duplex preparation aminoethylcarbamate linker ligation, amino linker, cross-link, labeling, nucleic acids, oligonucleotides, anti-microrna, Carbohydrates: Nucleic Acid Chemical Synthesis and other aspects.Application of 79642-50-5
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