On March 1, 2013, Dettmer, Ulf; Newman, Andrew J.; Luth, Eric S.; Bartels, Tim; Selkoe, Dennis published an article.Related Products of 79642-50-5 The title of the article was In Vivo Cross-linking Reveals Principally Oligomeric Forms of α-Synuclein and β-Synuclein in Neurons and Non-neural Cells. And the article contained the following:
Aggregation of α-synuclein (αSyn) in neurons produces the hallmark cytopathol. of Parkinson disease and related synucleinopathies. Since its discovery, αSyn has been thought to exist normally in cells as an unfolded monomer. It was recently reported that αSyn can instead exist in cells as a helically folded tetramer that resists aggregation and binds lipid vesicles more avidly than unfolded recombinant monomers. However, a subsequent study again concluded that cellular αSyn is an unfolded monomer. Here a simple in vivo crosslinking method is described that reveals a major ∼60-kDa form of endogenous αSyn monomer, 14.5 kDa in intact cells and smaller amounts of ∼80- and ∼100-kDa forms with the same isoelec. point as the 60-kDa species. Controls indicate that the apparent 60-kDa tetramer exists normally and does not arise from pathol. aggregation. The pattern of a major 60-kDa and minor 80- and 100-kDa species plus variable amounts of free monomers occurs endogenously in primary neurons and erythroid cells as well as neuroblastoma cells over-expressing αSyn. A similar pattern occurs for the homolog, β-synuclein, which does not undergo pathogenic aggregation. Cell lysis destabilizes the apparent 60-kDa tetramer, leaving mostly free monomers and some 80-kDa oligomer. However, lysis at high protein concentrations allows partial recovery of the 60-kDa tetramer. Together with the prior findings, these data suggest that endogenous αSyn exists principally as a 60-kDa tetramer in living cells but is lysis-sensitive, making the study of natural αSyn challenging outside of intact cells. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Related Products of 79642-50-5
The Article related to endogenous quaternary structure alpha beta synuclein oligomer crosslinking, General Biochemistry: Proteins and Their Constituents and other aspects.Related Products of 79642-50-5
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