On December 31, 2019, Wei, Ping; Guo, Ze-Wang; Wu, Xiao-Ling; Liang, Shan; Ou, Xiao-Yang; Xu, Pei; Zong, Min-Hua; Yang, Ji-Guo; Lou, Wen-Yong published an article.Safety of (R)-Methyl 3-hydroxybutanoate The title of the article was Significantly enhancing the biocatalytic synthesis of chiral alcohols by semi-rationally engineering an anti-Prelog carbonyl reductase from Acetobacter sp. CCTCC M209061. And the article contained the following:
Chiral alcs. and their derivatives are vital building blocks to synthesize pharmaceutical drugs and high-valued chems. Wild-type carbonyl reductase AcCR from Acetobacter sp. has ideal enantioselectivity toward 11 prochiral substrates (e.e.>99%) but poor activity. In this work, a semi-rational engineering was performed to enhance the activity of AcCR. Fortunately, three pos. double-mutants (mut-E144A/G152L, mut-G152L/Y189 N, and mut-I147 V/G152L) with specific activity 17-61 folds higher than that of enzyme without modified were achieved. Kinetic studies suggested that the catalytic efficiencies (kcat/Km) of these mutants were also well enhanced. Finally, these modified mut-AcCRs were successfully applied in asym. reductions of 11 structurally diverse prochiral substrates (200 mM) with excellent product yields (76.8%-99.1%) and enantiomeric excess (e.e.>99%), which provides an alternative strategy for efficient synthesis of chiral alcs. for pharmaceuticals industry with ideal yield and enantioselectivity. The experimental process involved the reaction of (R)-Methyl 3-hydroxybutanoate(cas: 3976-69-0).Safety of (R)-Methyl 3-hydroxybutanoate
The Article related to carbonyl reductase chiral alc synthesis, Fermentation and Bioindustrial Chemistry: Industrial Chemicals and other aspects.Safety of (R)-Methyl 3-hydroxybutanoate
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