Siedler, Solvej; Lindner, Steffen N.; Bringer, Stephanie; Wendisch, Volker F.; Bott, Michael published an article in 2013, the title of the article was Reductive whole-cell biotransformation with Corynebacterium glutamicum: improvement of NADPH generation from glucose by a cyclized pentose phosphate pathway using pfkA and gapA deletion mutants.Related Products of 3976-69-0 And the article contains the following content:
In this study, the potential of Corynebacterium glutamicum for reductive whole-cell biotransformation is shown. The NADPH-dependent reduction of the prochiral Me acetoacetate (MAA) to the chiral (R)-Me 3-hydroxybutyrate (MHB) by an alc. dehydrogenase from Lactobacillus brevis (Lbadh) was used as model reaction and glucose served as substrate for the regeneration of NADPH. Since NADPH is mainly formed in the oxidative branch of the pentose phosphate pathway (PPP), C. glutamicum was engineered to redirect carbon flux towards the PPP. Mutants lacking the genes for 6-phosphofructokinase (pfkA) or glyceraldehyde 3-phosphate dehydrogenase (gapA) were constructed and analyzed with respect to growth, enzyme activities, and biotransformation performance. Both mutants showed strong growth defects in glucose minimal medium. For biotransformation of MAA to MHB using glucose as reductant, strains were transformed with an Lbadh expression plasmid. The wild type showed a specific MHB production rate of 3.1 mmolMHB h-1 g and a yield of 2.7 molMHB mol. The ΔpfkA mutant showed a similar MHB production rate, but reached a yield of 4.8 molMHB mol, approaching the maximal value of 6 molNADPH mol expected for a partially cyclized PPP. The specific biotransformation rate of the ΔgapA mutant was decreased by 62 % compared to the other strains, but the yield was increased to 7.9 molMHB mol, which to our knowledge is the highest one reported so far for this mode of NADPH regeneration. As one fourth of the glucose was converted to glycerol, the exptl. yield was close to the theor. maximal yield of 9 molNADPH mol. The experimental process involved the reaction of (R)-Methyl 3-hydroxybutanoate(cas: 3976-69-0).Related Products of 3976-69-0
The Article related to corynebacterium nadph generation reductive biotransformation pfka gapa deletion, Fermentation and Bioindustrial Chemistry: Industrial Chemicals and other aspects.Related Products of 3976-69-0
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics