On March 22, 2017, Mandal, Satadru Sekhar; Duncombe, Lucy; Ganesh, N. Vijaya; Sarkar, Susmita; Howells, Laurence; Hogarth, Philip J.; Bundle, David R.; McGiven, John published an article.SDS of cas: 79642-50-5 The title of the article was Novel solutions for vaccines and diagnostics to combat brucellosis. And the article contained the following:
Brucellosis is diagnosed by detection of antibodies in the blood of animals and humans that are specific for two carbohydrate antigens, termed A and M, which are present concurrently in a single cell wall O-polysaccharide. Animal brucellosis vaccines contain these antigenic determinants, and consequently infected and vaccinated animals cannot be differentiated as both groups produce A and M specific antibodies. We hypothesized that chem. synthesis of a pure A vaccine would offer unique identification of infected animals by a synthetic M diagnostic antigen that would not react with antibodies generated by this vaccine. Two forms of the A antigen, a hexasaccharide and a heptasaccharide conjugated to tetanus toxoid via reducing and nonreducing terminal sugars, were synthesized and used as lead vaccine candidates. Mouse antibody profiles to these immunogens showed that to avoid reaction with diagnostic M antigen it was essential to maximize the induction of anti-A antibodies that bind internal oligosaccharide sequences and minimize production of antibodies directed toward the terminal nonreducing monosaccharide. This objective was achieved by conjugation of Brucella O-polysaccharide to tetanus toxoid via its periodate oxidized terminal nonreducing monosaccharide, thereby destroying terminal epitopes and focusing the antibody response on internal A epitopes. This establishes the method to resolve the decades-long challenge of how to create effective brucellosis vaccines without compromising diagnosis of infected animals. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).SDS of cas: 79642-50-5
The Article related to vaccine brucella a antigen polysaccharide tetanus toxoid brucellosis, diagnosis brucellosis brucella synthetic m antigen polysaccharide, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.SDS of cas: 79642-50-5
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