On November 1, 2015, Kukacka, Zdenek; Rosulek, Michal; Strohalm, Martin; Kavan, Daniel; Novak, Petr published an article.Application of 79642-50-5 The title of the article was Mapping protein structural changes by quantitative cross-linking. And the article contained the following:
Chem. crosslinking is a promising technol. for protein tertiary structure determination Though the data has low spatial resolution, it is possible to obtain it at physiol. conditions on proteins that are not amenable to standard high resolution techniques such as X-ray, NMR anal. and cryo-EM. Here we demonstrate the utilization of isotopically labeled chem. crosslinking to visualize protein conformation rearrangements. Since calmodulin exists in two distinct conformations (calcium-free and calcium-containing forms), we selected this protein for testing the potential and the limits of a new technique. After crosslinking of both calmodulin forms, the calcium-free and calcium-containing forms were mixed together and digested under different conditions and the products of proteolysis were monitored using high resolution mass spectrometry. Finally, the ratios of heavy/light cross-links were calculated by mMass open source platform. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5
The Article related to protein mapping structural change quant cross linking, chemical cross-linking, mass spectrometry, protein structure design, proteolysis, quantification, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 79642-50-5
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