On August 31, 1985, Felix, Arthur M.; Heimer, Edgar P.; Wang, Ching Tso; Lambros, Theodore J.; Swistok, Joseph; Roszkowski, Martin; Ahmad, Mustaq; Confalone, Dianne; Scott, John W. published an article.Product Details of 53838-27-0 The title of the article was Synthesis of thymosin α1 by fragment condensation using tert-butyl side chain protection. And the article contained the following:
Thymosin α1 (I) was prepared by classical methods using 7 tert-Bu side chain protected fragments. Optimum conditions were found for the final DCC/HOBt coupling of the two key intermediates; decapeptide and octadecapeptide. I was purified by two stages of preparative HPLC (partial purification with C8 and final purification with C18 reverse phase silica gel) to give a 30% overall yield for the final four stages of synthesis (including catalytic hydrogenation of octadecapeptide, coupling, deprotection and purification). The product was homogeneous according to thin-layer and paper high voltage electrophoresis, isoelec. focusing anal., thin-layer chromatog. and high-performance liquid chromatog. Amino acid anal., optical rotation, 1H NMR spectroscopy, FAB mass spectroscopy and peptide mapping after tryptic digestion confirmed the structure of thymosin α1. Three minor stereoisomer contaminants were isolated by HPLC and characterized as [D-Lys14]-thymosin α1, [D-Lys17]-thymosin α1 and [D-Ala3]-thymosin α1 resulting from racemization at Lys14, Lys17 and Ala3 during the coupling of the fragments. A final contaminant, isolated by HPLC, was characterized as Nα-isobutyloxycarbonyl-thymosin α1 (15-28), which results from “wrong way opening” of an activated mixed anhydride. The experimental process involved the reaction of (S)-5-tert-Butyl 1-methyl 2-aminopentanedioate(cas: 53838-27-0).Product Details of 53838-27-0
The Article related to thymosin synthesis fragment condensation, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Product Details of 53838-27-0
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