On November 7, 2018, Bhushan, Bhaskar; Lin, Yuya A.; Bak, Martin; Phanumartwiwath, Anuchit; Yang, Nan; Bilyard, Matthew K.; Tanaka, Tomonari; Hudson, Kieran L.; Lercher, Lukas; Stegmann, Monika; Mohammed, Shabaz; Davis, Benjamin G. published an article.Category: esters-buliding-blocks The title of the article was Genetic Incorporation of Olefin Cross-Metathesis Reaction Tags for Protein Modification. And the article contained the following:
Olefin cross-metathesis (CM) is a viable reaction for the modification of alkene-containing proteins. Although allyl sulfide or selenide side-chain motifs in proteins can critically enhance the rate of CM reactions, no efficient method for their site-selective genetic incorporation into proteins has been reported to date. Here, through the systematic evaluation of olefin-bearing unnatural amino acids for their metabolic incorporation, we have discovered S-allylhomocysteine (Ahc) as a genetically encodable Met analog that is not only processed by translational cellular machinery but also a privileged CM substrate residue in proteins. In this way, Ahc was used for efficient Met codon reassignment in a Met-auxotrophic strain of E. coli (B834 (DE3)) as well as metabolic labeling of protein in human cells and was reactive toward CM in several representative proteins. This expands the use of CM in the toolkit for “tag-and-modify” functionalization of proteins. The experimental process involved the reaction of 3-Aminodihydrothiophen-2(3H)-one hydrochloride(cas: 6038-19-3).Category: esters-buliding-blocks
The Article related to genetic incorporation olefin cross metathesis reaction tag protein modification, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Category: esters-buliding-blocks
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