Santos, Luiz F. A.; Iglesias, Amadeu H.; Gozzo, Fabio C. published an article in 2011, the title of the article was Fragmentation features of intermolecular cross-linked peptides using N-hydroxy- succinimide esters by MALDI- and ESI-MS/MS for use in structural proteomics.Electric Literature of 79642-50-5 And the article contains the following content:
The use of mass spectrometry coupled with chem. crosslinking of proteins has become one of the most useful tools for proteins structure and interactions studies. One of the challenges in these studies is the identification of the cross-linked peptides. The interpretation of the MS/MS data generated in crosslinking experiments using N-hydroxy succinimide esters is not trivial once a new amide bond is formed allowing new fragmentation pathways, unlike linear peptides. Intermol. cross-linked peptides occur when two different peptides are connected by the cross-linker and they yield information on the spatial proximity of different domains (within a protein) or proteins (within a complex). In this article, the authors report a detailed fragmentation study of intermol. cross-linked peptides, generated from a set of synthetic peptides, using both ESI and MALDI to generate the precursor ions. The fragmentation features observed here can be helpful in the interpretation and identification of cross-linked peptides present in crosslinking experiments and be further implemented in search engine’s algorithms. Copyright © 2011 John Wiley & Sons, Ltd. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5
The Article related to maldi mass spectrometry fragmentation intermol crosslinked peptide hydroxysuccinimide ester, electrospray ionization mass spectrometry fragmentation intermol crosslinked peptide hydroxysuccinimide and other aspects.Electric Literature of 79642-50-5
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