On December 31, 2019, Rao, Jingxin; Zhang, Rongzhen; Liang, Hongbo; Gao, Xiao-Dong; Nakanishi, Hideki; Xu, Yan published an article.Electric Literature of 3976-69-0 The title of the article was Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents. And the article contained the following:
Background: Saccharomyces cerevisiae AN120 osw2Δ spores were used as a host with good resistance to unfavorable environment. This work was undertaken to develop a new yeast spore-encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase for efficient chiral synthesis in organic solvents. Results: The spore microencapsulation of E228S/SCR II and GDH in S. cerevisiae AN120 osw2Δ catalyzed (R)-phenylethanol in a good yield with an excellent enantioselectivity (up to 99%) within 4 h. It presented good resistance and catalytic functions under extreme temperature and pH conditions. The encapsulation produced several chiral products with over 70% yield and over 99% enantioselectivity in Et acetate after being recycled for 4-6 times. It increased substrate concentration over threefold and reduced the reaction time two to threefolds compared to the recombinant Escherichia coli containing E228S and glucose dehydrogenase. Conclusions: This work first described sustainable enantioselective synthesis without exogenous cofactors in organic solvents using yeast spore-microencapsulation of coupled alc. dehydrogenases.[Figure not available: see fulltext.]. The experimental process involved the reaction of (R)-Methyl 3-hydroxybutanoate(cas: 3976-69-0).Electric Literature of 3976-69-0
The Article related to saccharomyces candida bacillus spore encapsulation carbonyl reductase gdh solvent, (s)-carbonyl reductase ii, glucose dehydrogenase, organic solvent, spore-microencapsulation, sustainable enantioselective catalysis and other aspects.Electric Literature of 3976-69-0
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