On April 30, 2020, Fiala, Jan; Kukacka, Zdenek; Novak, Petr published an article.Related Products of 79642-50-5 The title of the article was Influence of cross-linker polarity on selectivity towards lysine side chains. And the article contained the following:
The combination of chem. crosslinking and mass spectrometry is currently a progressive technol. for deriving structural information of proteins and protein complexes. In addition, chem. crosslinking is a powerful tool for stabilizing macromol. complexes for single particle cryo-electron microscopy. However, the effect of cross-linker polarity on the outcome of crosslinking reaction has never been studied. Herein, we use both polar (bis(sulfosuccinimidyl) glutarate) and non-polar (disuccinimidyl glutarate) cross-linkers and systematically investigated the impact of cross-linker hydrophobicity on resulting distance constraints, using bovine serum albumin as a model protein. Even though the amine reactive BS2G and DSG cross-linkers have the same length of spacer and are based on N-hydroxysuccinimidic group, our data showed that each of them formed preferentially different cross-links. We demonstrated that the choice of cross-linker can have a significant impact on the output data for structural characterization of biomols. Using equimolar mixtures of DSG with d6-BS2G, and BS2G with d6-DSG, we established that the polar BS2G preferentially bound to polar regions of modified mol., whereas non-polar DSG bound to hydrophobic regions. This phenomenon established that the mixture of polar and non-polar cross-linkers acted as an efficient tool for the determination of distance constraints in proteins. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Related Products of 79642-50-5
The Article related to protein structure lysine cross linker polarity mass spectrometry, bis(sulfosuccinimidyl) glutarate, bovine serum albumin, chemical cross-linking, disuccinimidyl glutarate, protein structure, structural mass spectrometry and other aspects.Related Products of 79642-50-5
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