Eldemerdash, Marwa M.; El-Sayed, Ashraf S. A.; Hussein, Hussein A.; Teleb, Samir S.; Shehata, Rania S. published the artcile< Molecular and metabolic traits of some Egyptian species of Cassia L. and Senna Mill (Fabaceae-Caesalpinioideae)>, Recommanded Product: (9Z,12Z)-Methyl octadeca-9,12-dienoate, the main research area is Cassia Senna metabolism Egypt; Cassia; GC-MS profile; ITS sequence; RAPD analysis; Senna; Taxonomical features.
The genus Cassia and Senna have been classified under subfamily Caesalpinioideae of family Fabaceae (Leguminosae) of order Fabales. There is a scarce taxonomical studies of the genus Cassia and Senna inhabiting Egyptian environments, thus, the main objective of the current was to revise and authenticate the phylogenetic relationship between studied taxa of the species of the genera Cassia and Senna in Egypt using the recent tools of ITS barcoding, RAPD anal. and metabolic profiling, in comparing to the traditional taxonomical features. From the cluster anal. of the traditional 27 morphol. characters, the studied taxa were categorized into two major clades with an average taxonomic distance of 4.3. The clade I include Cassia fistula, C. renigera, C. javanica L subsp. nodosa and C. roughiia that belongs to series Obolospermae, and C. grandis that belongs to series Grandes. The clade (II) includes Senna surattensis and S. alata at taxonomic level 3.6. The taxonomical description of the studied taxa was confirmed from the mol. anal. of ITS sequences and RAPD anal. The ITS sequences of the tested plants species C. fistula L, C. grandis MD4, C. javanica subsp. nodosa MD7, C. roxburghii MD5, C. renigera MD5 were deposited at genbank with accession numbers MW367973, MZ960447, MW386305, MW326753 and MW32685, resp. While, the ITS sequences of the S. surrattensis and S. alata were deposited into genbank accession # MD14 MW367670 and MD20 MW412635, resp. Thus, from the mol. anal., two clades were clearly separated into Clade I of Cassia and Clade II of Senna. The cluster I represented by C. fistula, C. renigera, C. roxburghii, and C. javanica sub nodosa, and the cluster II represented by S. alata and S. surattensis. From the PCA of RAPD, a clearly discrimination between the two Taxa was observed revealing the characteristic grouping of Cassia and Senna. The species Senna alata and Senna surattensis were grouped together, but the species of C. renigera, C. javanica, C. roxburghii and C. grandis was grouped on a distinct group. The separation of Cassia and Senna species into two clusters verify the segregation of the genus Cassia L. senso lato into two distinct genera namely Senna P. and Cassia L. The morphol., mol. traits of the studied plants were authenticated from the metabolic profiling by GC-MS anal. Among the 23 identified metabolites, four compounds namely hexadecanoic acid, Me ester, 9-Octadecenoic acid (Z)-Et ester and Vitamin E were detected with fluctuated concentrations, among C. fistula, C. grandis, C. javanica subsp. nodosa and C. roxburghii. Conclusively, the traditional morphol. features, mol. barcoding using ITS sequences, RAPD anal. and metabolic traits by GC-MS anal., authenticates the taxonomical diversity of the genus Cassia and Senna.
BMC Plant Biology published new progress about Caesalpinioideae. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Recommanded Product: (9Z,12Z)-Methyl octadeca-9,12-dienoate.
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