Fan, Pei-Ru; Zhao, Xue; Wei, Ze-Hui; Huang, Yan-Ping; Liu, Zhao-Sheng published the artcile< Robust immobilized enzyme reactor based on trimethylolpropane trimethacrylate organic monolithic matrix through ""thiol-ene"" click reaction>, Application In Synthesis of 3290-92-4, the main research area is immobilized enzyme reactor trimethylolpropane trimethacrylate organic monolithic matrix.
A novel immobilized enzyme reactor (IMER) based on trimethylolpropane trimethacrylate (TRIM) organic monolith was prepared by “”thiol-ene”” click reaction. The IMERs were made by fabricating TRIM monolith core in advance. There were residual double bonds on the surface of TRIM monolith, in which click reaction can be performed with the free thiol groups of trypsin at room temperature to bond the trypsin on the monolithic column without addnl. introducing an active functional group. The enzyme activity was characterized by kinetic parameters Km and Vmax and determined by off-line chromatog. The performance of the IMERs was evaluated by digesting standard protein BSA and compared with in-solution digestion method. By using IMER 5 min, the sequence coverage rate of BSA was 79.41%, which was similar to that in-solution digestion for 12 h (82.7%). The applicability of the IMERs in complex samples was assessed by digesting crude protein extracts from egg white and mouse liver, identifying 28 (104 peptides) and 843 (3916 peptides) proteins, resp. All results indicated that the IMERs have great potential in high-throughput proteomics anal.
European Polymer Journal published new progress about Bioreactors. 3290-92-4 belongs to class esters-buliding-blocks, and the molecular formula is C18H26O6, Application In Synthesis of 3290-92-4.
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