Ossowicz, Paula; Kardaleva, Proletina; Guncheva, Maya; Klebeko, Joanna; Swiatek, Ewelina; Janus, Ewa; Yancheva, Denitsa; Angelov, Ivan published the artcile< Ketoprofen-based ionic liquids: synthesis and interactions with bovine serum albumin>, Application In Synthesis of 2743-40-0, the main research area is ketoprofen based ionic liquid formulation bovine serum albumin; binding constants; bovine serum albumin; ionic liquids; ketoprofen; secondary structure.
The development of ionic liquids based on active pharmaceutical ingredients (API-ILs) is a possible solution to some of the problems of solid and/or hydrophobic drugs such as low solubility and bioavailability, polymorphism and an alternative route of administration could be suggested as compared to the classical drug. Here, we report for the first time the synthesis and detailed characterization of a series of ILs containing a cation amino acid esters and anion ketoprofen (KETO-ILs). The affinity and the binding mode of the KETO-ILs to bovine serum albumin (BSA) were assessed using fluorescence spectroscopy. All compounds bind in a distance not longer than 6.14 nm to the BSA fluorophores. The estimated binding constants (KA) are in order of 105 L mol-1, which is indicative of strong drug or IL-BSA interactions. With respect to the ketoprofen-BSA system, a stronger affinity of the ILs containing l-LeuOEt, l-ValOBu, and l-ValOEt cation towards BSA is clearly seen. Fourier transformed IR spectroscopy experiments have shown that all studied compounds induced a rearrangement of the protein mol. upon binding, which is consistent with the suggested static mechanism of BSA fluorescence quenching and formation of complexes between BSA and the drugs. All tested compounds were safe for macrophages.
Molecules published new progress about Bovine serum albumin Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2743-40-0 belongs to class esters-buliding-blocks, and the molecular formula is C8H18ClNO2, Application In Synthesis of 2743-40-0.
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