Singh, YashoNandini; Rodriguez Benavente, Maria C.; Al-Huniti, Mohammed H.; Beckwith, Donella; Ayyalasomayajula, Ramya; Patino, Eric; Miranda, William S.; Wade, Alex; Cudic, Mare published the artcile< Positional Scanning MUC1 Glycopeptide Library Reveals the Importance of PDTR Epitope Glycosylation for Lectin Binding>, Electric Literature of 4098-06-0, the main research area is MUC1 glycopeptide epitope combinatorial library lectin binding cancer glycosylation.
One of the main barriers to explaining the functional significance of glycan-based changes in cancer is the natural epitope heterogeneity found on the surface of cancer cells. To help address this knowledge gap, we focused on designing synthetic tools to explore the role of tumor-associated glycans of MUC1 in the formation of metastasis via association with lectins. In this study, we have synthesized for the first time a MUC1-derived positional scanning synthetic glycopeptide combinatorial library (PS-SGCL) that vary in number and location of cancer-associated Tn antigen using the “”tea bag”” approach. The determination of the isokinetic ratios necessary for the equimolar incorporation of (glyco)amino acids mixtures to resin-bound amino acid was determined, along with developing an efficient protocol for on resin deprotection of O-acetyl groups. Enzyme-linked lectin assay (ELLA) was used to screen PS-SGCL against two plant lectins, Glycine max soybean agglutinin (SBA) and Vicia villosa (VVA). Results revealed a carbohydrate d.-dependent affinity trend and site-specific glycosylation requirements for high affinity binding to these lectins. Hence, PS-SGCLs provide a platform to systematically elucidate MUC1-lectin binding specificities, which in long term may provide a rational design for novel inhibitors of MUC1-lectin interactions involved in tumor spread and glycopeptide-based cancer vaccines.
Journal of Organic Chemistry published new progress about Agglutinins Role: ARU (Analytical Role, Unclassified), BSU (Biological Study, Unclassified), ANST (Analytical Study), BIOL (Biological Study) (soybean). 4098-06-0 belongs to class esters-buliding-blocks, and the molecular formula is C12H16O7, Electric Literature of 4098-06-0.
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