Teixeira, Priscila Camillo; Ducret, Axel; Langen, Hanno; Nogoceke, Everson; Santos, Ronaldo Honorato Barros; Nunes, Joao Paulo Silva; Benvenuti, Luiz; Levy, Debora; Bydlowski, Sergio Paulo; Bocchi, Edimar Alcides; Takara, Andreia Kuramoto; Fiorelli, Alfredo Inacio; Stolf, Noedir Antonio; Pomeranzeff, Pablo; Chevillard, Christophe; Kalil, Jorge; Cunha-Neto, Edecio published an article in 2021, the title of the article was Impairment of multiple mitochondrial energy metabolism pathways in the heart of chagas disease cardiomyopathy patients.Category: esters-buliding-blocks And the article contains the following content:
Chagas disease cardiomyopathy (CCC) is an inflammatory dilated cardiomyopathy occurring in 30% of the 6 million infected with the protozoan Trypanosoma cruzi in Latin America. Survival is significantly lower in CCC than ischemic (IC) and idiopathic dilated cardiomyopathy (DCM). Previous studies disclosed a selective decrease in mitochondrial ATP synthase alpha expression and creatine kinase activity in CCC myocardium as compared to IDC and IC, as well as decreased in vivo myocardial ATP production Aiming to identify addnl. constraints in energy metabolism specific to CCC, we performed a proteomic study in myocardial tissue samples from CCC, IC and DCM obtained at transplantation, in comparison with control myocardial tissue samples from organ donors. Left ventricle free wall myocardial samples were subject to two-dimensional electrophoresis with fluorescent labeling (2D-DIGE) and protein identification by mass spectrometry. We found altered expression of proteins related to mitochondrial energy metabolism, cardiac remodeling, and oxidative stress in the 3 patient groups. Pathways anal. of proteins differentially expressed in CCC disclosed mitochondrial dysfunction, fatty acid metabolism and transmembrane potential of mitochondria. CCC patients’ myocardium displayed reduced expression of 22 mitochondrial proteins belonging to energy metabolism pathways, as compared to 17 in DCM and 3 in IC. Significantly, 6 beta-oxidation enzymes were reduced in CCC, while only 2 of them were down-regulated in DCM and 1 in IC. We also observed that the cytokine IFN-gamma, previously described with increased levels in CCC, reduces mitochondrial membrane potential in cardiomyocytes. Results suggest a major reduction of mitochondrial energy metabolism and mitochondrial dysfunction in CCC myocardium which may be in part linked to IFN-gamma. This may partially explain the worse prognosis of CCC as compared to DCM or IC. The experimental process involved the reaction of Oxybis(ethane-2,1-diyl) bis(2-methylacrylate)(cas: 2358-84-1).Category: esters-buliding-blocks
The Article related to mitochondrial energy metabolism pathway chagas disease cardiomyopathy human, chronic chagas disease cardiomyopathy, energy metabolism, idiopathic dilated cardiomyopathy, interferon-gamma, ischemic cardiomyopathy, mitochondria, proteomics, two-dimensional electrophoresis with fluorescent labeling and other aspects.Category: esters-buliding-blocks
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