On August 10, 2011, Hu, Mingyu; Li, Lin; Wu, Hao; Su, Ying; Yang, Peng-Yu; Uttamchandani, Mahesh; Xu, Qing-Hua; Yao, Shao Q. published an article.Formula: C12H15NO4 The title of the article was Multicolor, one- and two-photon imaging of enzymatic activities in live cells with fluorescently quenched activity-based probes (qABPs). And the article contained the following:
Fluorescence imaging provides an indispensable way to locate and monitor biol. targets within complex and dynamic intracellular environments. Of the various imaging agents currently available, small mol.-based probes provide a powerful tool for live cell imaging, primarily due to their desirable properties, including cell permeability (as a result of their smaller sizes), chem. tractability (e.g., different mol. structures/designs can be installed), and amenability to imaging a wide variety of biol. events. With a few exceptions, most existing small mol. probes are however not suitable for in vivo bioimaging experiments in which high-resolution studies of enzyme activity and localization are necessary. Here, the authors report a new class of fluorescently quenched activity-based probes (qABPs) which are highly modular, and can sensitively image (through multiple enzyme turnovers leading to fluorescence signal amplification) different types of enzyme activities in live mammalian cells with good spatial and temporal resolution The authors also incorporated 2-photon dyes into their modular probe design, enabling for the 1st time activity-based, fluorogenic 2-photon imaging of enzyme activities. Thus, this expands the repertoire of ‘smart’, responsive probes currently available for live cell bioimaging experiments The experimental process involved the reaction of tert-Butyl 2-(4-nitrophenyl)acetate(cas: 29704-38-9).Formula: C12H15NO4
The Article related to enzyme imaging live cell fluorescently quenched activity based probe, caspase imaging live cell fluorescently quenched activity based probe, phosphatase imaging live cell fluorescently quenched activity based probe, fluorescently quenched activity based probe preparation enzyme imaging cell and other aspects.Formula: C12H15NO4
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