In 2018,Yang, Xue; Michiels, Thomas J. M.; de Jong, Coen; Soethoudt, Marjolein; Dekker, Niek; Gordon, Euan; van der Stelt, Mario; Heitman, Laura H.; van der Es, Daan; IJzerman, Adriaan P. published 《An Affinity-Based Probe for the Human Adenosine A2A Receptor》.Journal of Medicinal Chemistry published the findings.Application In Synthesis of tert-Butyl (5-aminopentyl)carbamate The information in the text is summarized as follows:
Using activity-based protein profiling (ABPP), functional proteins can be interrogated in their native environment. Despite their pharmaceutical relevance, G protein-coupled receptors (GPCRs) have been difficult to address through ABPP. In the current study, the authors took the prototypical human adenosine A2A receptor (hA2AR) as the starting point for the construction of a chem. toolbox allowing two-step affinity-based labeling of GPCRs. First, the authors equipped an irreversibly binding hA2AR ligand with a terminal alkyne to serve as probe. The authors showed that the probe irreversibly and concentration-dependently labeled purified hA2AR. Click-ligation with a sulfonated cyanine-3 fluorophore allowed us to visualize the receptor on SDS-PAGE. The authors further demonstrated that labeling of the purified hA2AR by the probe could be inhibited by selective antagonists. Lastly, the authors showed successful labeling of the receptor in cell membranes overexpressing hA2AR, making the probe a promising affinity-based tool compound that sets the stage for the further development of probes for GPCRs. The experimental process involved the reaction of tert-Butyl (5-aminopentyl)carbamate(cas: 51644-96-3Application In Synthesis of tert-Butyl (5-aminopentyl)carbamate)
Some of the reported applications of tert-Butyl (5-aminopentyl)carbamate(cas: 51644-96-3) include: synthesis of of a supermacrocycle that self-assemble to form organic nanotubes., preparation of water-soluble unsymmetrical sulforhodamine fluorophores from monobrominated sulfoxanthene dye, synthesis of functionalized porphyrins as biocompatible carrier system for photodynamic therapy (PDT).Application In Synthesis of tert-Butyl (5-aminopentyl)carbamate
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