Petrovicova, Tatiana; Gyuranova, Dominika; Plz, Michal; Myrtollari, Kamela; Smonou, Ioulia; Rebros, Martin published an article in 2021. The article was titled 《Application of robust ketoreductase from Hansenula polymorpha for the reduction of carbonyl compounds》, and you may find the article in Molecular Catalysis.Product Details of 609-14-3 The information in the text is summarized as follows:
Enzyme-catalyzed asym. reduction of ketones is an attractive tool for the production of chiral building blocks or precursors for the synthesis of bioactive compounds Expression of robust ketoreductase (KRED) from Hansenula polymorpha was upscaled and applied for the asym. reduction of 31 prochiral carbonyl compounds (aliphatic and aromatic ketones, diketones and β-keto esters) to the corresponding optically pure hydroxy compounds Biotransformations were performed with the purified recombinant KRED together with NADP+ recycling glucose dehydrogenase (GDH, Bacillus megaterium), both overexpressed in Escherichia coli BL21(DE3). Maximum activity of KRED for biotransformation of ethyl-2-methylacetoacetate achieved by the high cell d. cultivation was 2499.7 ± 234 U g-1DCW and 8.47 ± 0.40 U·mg-1E, resp. The KRED from Hansenula polymorpha is a very versatile enzyme with broad substrate specificity and high activity towards carbonyl substrates with various structural features. Among the 36 carbonyl substrates screened in this study, the KRED showed activity with 31, with high enantioselectivity in most cases. With several ketones, the Hansenula polymorpha KRED catalyzed preferentially the formation of the (R)-secondary alcs., which is highly valued. In addition to this study using Ethyl 2-methyl-3-oxobutanoate, there are many other studies that have used Ethyl 2-methyl-3-oxobutanoate(cas: 609-14-3Product Details of 609-14-3) was used in this study.
Ethyl 2-methyl-3-oxobutanoate(cas: 609-14-3) belongs to ketone compounds. They are most widely used as solvents, especially in industries manufacturing explosives, lacquers, paints, and textiles. Ketones are also used in tanning, as preservatives, and in hydraulic fluids.Product Details of 609-14-3
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics