Chien, Tiffany; Jones, Drew R.; Danino, Tal published an article in 2021. The article was titled 《Engineered bacterial production of volatile me salicylate》, and you may find the article in ACS Synthetic Biology.Reference of Methyl Salicylate The information in the text is summarized as follows:
The engineering of microbial metabolic pathways over the last two decades has led to numerous examples of cell factories used for the production of small mols. These mols. have an array of utility in com. industries and as in situ expressed biomarkers or therapeutics in microbial applications. While most efforts have focused on the production of mols. in the liquid phase, there has been increasing interest in harnessing microbes’ inherent ability to generate volatile compounds Here, we optimized and characterized the production of Me salicylate, an aromatic compound found mainly in plants, using a common lab strain of E. coli. We utilized genetic components from both microbes and plants to construct the volatile metabolite circuit cassette. In order to maximize production, we explored expression of Me salicylate precursors, upregulation of expression by increasing ribosomal binding strength and codon optimization of the Me transferase gene obtained from plant Petunia x hybrida. Last, we validated and quantified the production of Me salicylate with liquid chromatog. or gas chromatog. mass spectrometry (LC-MS or GC-MS) and found that the codon optimized strain with precursor supplementation yielded the highest production compared to the other strains. This work characterizes an optimized metabolite producing genetic circuit and sets the stage for creation of an engineered bacteria diagnostic to be used in volatile assays. In the experiment, the researchers used many compounds, for example, Methyl Salicylate(cas: 119-36-8Reference of Methyl Salicylate)
Methyl Salicylate(cas: 119-36-8) has been used: as a component of clarifying solution for treating Mongolian gerbil cochlea intact for immunofluorescence analysis, as a plant elicitor to test its effect on reducing the whitefly population from tomato plants.Reference of Methyl Salicylate
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Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics