Formula: C11H24N2O2In 2020 ,《Regioselective SN2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation》 appeared in ACS Applied Bio Materials. The author of the article were Adak, Avijit K.; Huang, Kuan-Ting; Li, Pei-Jhen; Fan, Chen-Yo; Lin, Po-Chiao; Hwang, Kuo-Chu; Lin, Chun-Cheng. The article conveys some information:
Antibodies have exquisite specificities for mol. recognition, which led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation control over antibody immobilization. Here, the authors report a mild electrophilic phenylsulfonate (tosylate) ester-containing boronic acid affinity ligand for attaching antibodies to glass slides. A high level of antibody coupling located near the Fc region of the boronated antibody complex could be achieved by the proximal nucleophilic amino acid driven substitution reaction at the phenylsulfonate center. This enabled the full-length antibodies to be permanently tethered onto surfaces in an oriented manner. The advantages of this strategy were demonstrated through the individual and multiplex detection of protein and serum biomarkers. This strategy not only confers stability to the immobilized antibodies but also presents a different direction for the irreversible attachment of antibodies to solid supports in an orientation-controlled way. In the experiment, the researchers used N-Boc-1,6-Diaminohexane(cas: 51857-17-1Formula: C11H24N2O2)
N-Boc-1,6-Diaminohexane(cas: 51857-17-1) is used to prepare 1,3-Bis[6-(Boc-amino)hexyl]urea by reacting with carbonyl dichloride in the presence of triethylamine. Further, it is used as a reagent for the introduction of a C6-spacer.Formula: C11H24N2O2
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