Kim, Min Kyoung; Park, Geonha; Hong, Seon-Pyo; Jang, Young Pyo published the artcile< Analytical quality by design methodology approach for simultaneous quantitation of paeoniflorin and decursin in herbal medicine by RP-HPLC analysis>, Electric Literature of 112-63-0, the main research area is paeoniflorin decursin herbal medicine RPHPLC quantitation.
Simultaneous quantification of multiple marker compounds in herbal medicine by high performance liquid chromatog. (HPLC) anal. is still a challenge due to the complexity in various parameters to be considered and co-existing multi-components. As a case study, a reliable HPLC method for simultaneous quantification of paeoniflorin from Paeoniae Radix and decursin from Angelicae Gigantis Radix in various com. herbal medicine was developed based on anal. quality by design (AQbD) strategy. As a first step, risk assessment was performed to select the critical method parameters (CMPs) which were decided as organic mobile phase ratio and column oven temperature In order to evaluate the effect of the CMPs on critical method attributes (CMAs) of peak resolution and tailing, central composite design (CCD) was employed. The final chromatog. conditions were optimized as follows: column- C18, 4.6 x 250 mm, 5μm particle size; mobile phase- A: acetonitrile, B: 0.1% acetic acid water; detection wavelength- 235 nm for paeoniflorin, 325 nm for decursin; column oven temperature- 25°C; flow rate- 1.0 mL/min; gradient mobile phase system as Time (min): % A, 0:14, 25:14, 30:50, 60:50, 61:100, 65:100, 66:14, 75:14. The method was successfully validated according to the International Conference on Harmonization (ICH) guidelines and piloted for ten com. herbal medicines.
Natural Product Sciences published new progress about Particle size. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Electric Literature of 112-63-0.
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