Esen, Cem; Czulak, Joanna; Cowen, Todd; Piletska, Elena; Piletsky, Sergey A. published the artcile< Highly Efficient Abiotic Assay Formats for Methyl Parathion: Molecularly Imprinted Polymer Nanoparticle Assay as an Alternative to Enzyme-Linked Immunosorbent Assay>, HPLC of Formula: 3290-92-4, the main research area is abiotic determination format methyl parathion molecularly imprinted polymer nanoparticle.
ELISA is a widely used standard method for sensitive detection of analytes of environmental, clin., or biotechnol. interest. However, ELISA has clear drawbacks related to the use of relatively unstable antibodies and enzyme conjugates and the need for several steps such as washing of nonbound conjugates and addition of dye reagents. Herein, the authors introduce a new completely abiotic assay where antibodies and enzymes are replaced with fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) and target-conjugated magnetic nanoparticles, which acted as both reporter probes and binding agents. The components of the molecularly imprinted polymer nanoparticle assay (MINA) are assembled in microtiter plates fitted with magnetic inserts. The authors have compared the performance of a new magnetic assay with molecularly imprinted polymer (MIP)-based ELISA for the detection of methyl parathion (MP). Both assays showed high sensitivity toward allowing detection of MP at picomolar concentrations without any cross-reactivity against chlorpyriphos and fenthion. The fully abiotic assays were also proven to detect analyte in real samples such as tap water and milk. Unlike ELISA-based systems, the novel assay required no washing steps or addition of enzyme substrates, making it more user-friendly and suitable for high throughput screening.
Analytical Chemistry (Washington, DC, United States) published new progress about Drinking waters. 3290-92-4 belongs to class esters-buliding-blocks, and the molecular formula is C18H26O6, HPLC of Formula: 3290-92-4.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics