《A universal one-pot assay strategy based on bio-inorganic cascade catalysts for different analytes by changing pH-dependent activity of enzymes on enzyme mimics》 was written by Zhang, Haijiao; Han, Lei; Li, Feng. Formula: C5H11NO2This research focused onuniversal assay bioinorganic catalyst pH dependent analyte enzyme activity. The article conveys some information:
In general, peroxidase mimics show optimal activity at acidic pH, while many enzymes work at physiol. pH. To realize one-pot colorimetric detection based on enzyme-nanozyme-coupled system, the usual strategy is to change the pH-dependent activity of nanozymes, which is difficult for most of nanozymes. Unlike common strategies, we changed the pH-dependent activity of enzymes rather than nanozymes. First, we facilely synthesized Co3O4 magnetic peroxidase mimics with uniform size, good dispersibility and superparamagnetism by using protein as templates at mild condition. The as-prepared Co3O4 magnetic nanoparticles (MNPs) were covered with the layers of protein, facilitating the further functionalization with enzymes. Exhilaratingly, we discovered that the immobilization on Co3O4 MNPs could regulated pH-dependent activity of enzymes, such as glucose oxidase (GOx) and glucoamylase (GA). Based on the overlapped pH range between peroxidase-like activity of Co3O4 MNPs and catalytic activity of GOx, the hybrid cascade catalysts were employed for a one-pot glucose detection, exhibiting wide detection range and good selectivity. Furthermore, GA and GOx were co-mobilized on Co3O4 MNPs to realize the one-pot colorimetric starch detection, confirming the universality of the proposed strategy. As an addnl. bonus, hybrid cascade catalysts could be expediently separated from the reaction solution under the magnetic field in order to rapidly terminate the reaction and recycle enzymes. This work would open an avenue for the various one-pot enzyme-nanozyme-coupled assay. In the experiment, the researchers used many compounds, for example, tert-Butyl carbamate(cas: 4248-19-5Formula: C5H11NO2)
tert-Butyl carbamate(cas: 4248-19-5) belongs to anime. Amines have a free lone pair with which they can coordinate to metal centers. Amine–metal bonds are weaker because amines are incapable of backbonding, but they are still important for sensing applications.While stronger than hydrogen bonds, amine–metal bonds are still weaker than both covalent and ionic bonds.Formula: C5H11NO2
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