Application In Synthesis of N-Boc-1,6-DiaminohexaneIn 2021 ,《Label-free detection of target proteins using peptide molecular wires as conductive supports》 was published in Sensors and Actuators, B: Chemical. The article was written by Puiu, Mihaela; Zamfir, Lucian-Gabriel; Danila, George Madalin; Papi, Francesco; Nativi, Cristina; Mirceski, Valentin; Bala, Camelia. The article contains the following contents:
We report on the electrochem. of a peptide mol. wire as a conductive support for ligand immobilization in biosensing assays. The helical 9-mer peptide, tagged at Nterminus with Methylene Blue (MB) and thiol-functionalized at Cterminus was anchored on a gold surface via gold/thiol chem. The helical peptide acts as mol. wire, mediating the two-step electron transfer (ET) from MB to the gold surface. The forward and backward square wave voltametric (SWV) components recorded in the presence of peptide wire were used to estimate the kinetic parameters of the electrode reaction. The simulated data matched the exptl. ones for the two-step sequential surface mechanism (EE), with the rate constant of the first step ksur,1 = 20 s-1 and the cathodic ET coefficient α1 = 0.55. The kinetic parameters of the second step were ksur,2 > 1000 s-1, and α2 = 0.5. Small ligands for high-mol. weight targets can be grafted on the peptide wire between the MB tag and the surface through covalent bonding. The binding of the target to the peptide-anchored ligand hampers the ET transfer from MB to the electrode surface, causing a decrease of the peak current. These findings were used further to develop an electrochem. peptide-based biosensor with signaling-off interrogation. The biosensor was tested against two relevant targets for medical diagnosis: the anti-tumor-associated carbohydrate antigen (α-Tn) antibody and the growth hormone secretagogue receptor (GHS-R1a), after ligand grafting. Both targets were detected in the nanomolar range with an overall assay time of 10 min. In the experiment, the researchers used many compounds, for example, N-Boc-1,6-Diaminohexane(cas: 51857-17-1Application In Synthesis of N-Boc-1,6-Diaminohexane)
N-Boc-1,6-Diaminohexane(cas: 51857-17-1) is used to prepare 1,3-Bis[6-(Boc-amino)hexyl]urea by reacting with carbonyl dichloride in the presence of triethylamine. Further, it is used as a reagent for the introduction of a C6-spacer.Application In Synthesis of N-Boc-1,6-Diaminohexane
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