Song, Yaoyao; Wang, Lei; Liu, Fengjuan; Jiao, Chengjin; Nan, Hao; Shen, Xiao; Chen, Hong; Li, Yifan; Lei, Beilei; Jiang, Jinglong; Chen, Peng; Xu, Quanle published their research in Journal of Agricultural and Food Chemistry in 2021. The article was titled 《β-Cyanoalanine Synthase Regulates the Accumulation of β-ODAP via Interaction with Serine Acetyltransferase in Lathyrus sativus》.Synthetic Route of C5H6O2 The article contains the following contents:
β-N-Oxalyl-L-α,β-diaminopropionic acid (β-ODAP), found in Lathyrus sativus at first, causes a neurol. disease, lathyrism, when over ingested in an unbalanced diet. Our previous research suggested that β-ODAP biosynthesis is related to sulfur metabolism In this study, β-cyanoalanine synthase (β-CAS) was confirmed to be responsible for β-ODAP biosynthesis via in vitro enzymic anal. LsCAS was found to be pyridoxal phosphate (PLP)-dependent via spectroscopic anal. and dual functional via enzymic activity anal. Generation of a M135T/M235S/S239T triple mutant of LsCAS, which are the key sites to control the ratio of CAS/cysteine synthase (CS) activity, switches reaction chem. to that of a CS. LsCAS interactions were further screened and verified via Y2H, BiFC and pull-down assay. It was suggested that LsSAT2 interacts and forms a cysteine regulatory complex (CRC) with LsCAS in mitochondria, which improves LsSAT while reduces LsCAS activities to affect β-ODAP content pos. These results provide new insights into the mol. regulation of β-ODAP content in L. sativus. In the experiment, the researchers used many compounds, for example, Ethyl propiolate(cas: 623-47-2Synthetic Route of C5H6O2)
Ethyl propiolate(cas: 623-47-2) is a clear colorless to pale yellow liquid that is soluble in ethanol, ether and chloroform. It an important organic chemical raw material and pharmaceutical intermediate. Ethyl propargylate is obtained by oxidation of propargyl alcohol to propargylic acid followed by esterification.Synthetic Route of C5H6O2
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Ester – an overview | ScienceDirect Topics