《Unexpected Preference of the E. coli Translation System for the Ester Bond during Incorporation of Backbone-Elongated Substrates》 was written by Sando, Shinsuke; Abe, Kenji; Sato, Nobuhiko; Shibata, Toshihiro; Mizusawa, Keigo; Aoyama, Yasuhiro. Recommanded Product: Methyl 3-hydroxypropanoateThis research focused ontranslation ribosome tRNA protein oligopeptide hydroxypropionic acid stereochem. The article conveys some information:
There have been recent advances in the ribosomal synthesis of various mols. composed of nonnatural ribosomal substrates. However, the ribosome has strict limitations on substrates with elongated backbones. Here, we show an unexpected loophole in the E. coli translation system, based on a remarkable disparity in its selectivity for β-amino/hydroxy acids. We challenged β-hydroxypropionic acid (β-HPA), which is less nucleophilic than β-amino acids but free from protonation, to produce a new repertoire of ribosome-compatible but main-chain-elongated substrates. PAGE anal. and mass-coupled S-tag assays of amber suppression experiments using yeast suppressor tRNAPheCUA confirmed the actual incorporation of β-HPA into proteins/oligopeptides. We investigated the side-chain effects of β-HPA and found that the side chain at position α and R stereochem. of the β-substrate is preferred and even notably enhances the efficiency of incorporation as compared to the parent substrate. These results indicate that the E. coli translation machinery can utilize main-chain-elongated substrates if the pKa of the substrate is appropriately chosen. In the experimental materials used by the author, we found Methyl 3-hydroxypropanoate(cas: 6149-41-3Recommanded Product: Methyl 3-hydroxypropanoate)
Methyl 3-hydroxypropanoate(cas: 6149-41-3) belongs to esters with low molecular weight are commonly used as fragrances and found in essential oils and pheromones. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Recommanded Product: Methyl 3-hydroxypropanoate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics