Miranda, Renata Rank; Oliveira, Anny Carolline Silva; Skytte, Lilian; Rasmussen, Kaare Lund; Kjeldsen, Frank published the artcile< Proteome-wide analysis reveals molecular pathways affected by AgNP in a ROS-dependent manner>, Application In Synthesis of 112-63-0, the main research area is proteome ROS bend3 NRF2 cytotoxicity; N-Acetyl L-cysteine; Silver nanoparticles; metal uptake; oxidative stress; proteomics.
The use of mass spectrometry-based proteomics has been increasingly applied in nanomaterials risk assessments as it provides a proteome-wide overview of the mol. disturbances induced by its exposure. Here, we used this technique to gain detailed mol. insights into the role of ROS as an effector of AgNP toxicity, by incubating Bend3 cells with AgNP in the absence or presence of an antioxidant N-acetyl L-cystein (NAC). ROS generation is a key player in AgNP-induced toxicity, as cellular homeostasis was kept in the presence of NAC. By integrating MS/MS data with bioinformatics tools, in the absence of NAC, we were able to pinpoint precisely which biol. pathways were affected by AgNP. Cells respond to AgNP-induced ROS generation by increasing their antioxidant pool, via NRF2 pathway activation. Addnl., cell proliferation-related pathways were strongly inhibited in a ROS-dependent manner. These findings reveal important aspects of the AgNP mechanism of action at the protein level.
Nanotoxicology published new progress about BEN domain-containing protein 3 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Application In Synthesis of 112-63-0.
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