Lohse, Jonas; Swier, Lotteke J. Y. M.; Oudshoorn, Ruben C.; Medard, Guillaume; Kuster, Bernhard; Slotboom, Dirk-Jan; Witte, Martin D. published the artcile< Targeted Diazotransfer Reagents Enable Selective Modification of Proteins with Azides>, Category: esters-buliding-blocks, the main research area is diazotransfer reagent protein azide label.
In chem. biol., azides are used to chem. manipulate target structures in a bioorthogonal manner for a plethora of applications ranging from target identification to the synthesis of homogeneously modified protein conjugates. While a variety of methods have been established to introduce the azido group into recombinant proteins, a method that directly converts specific amino groups in endogenous proteins is lacking. Here, the authors report the first biotin-tethered diazotransfer reagent DtBio and demonstrate that it selectively modifies the model proteins streptavidin and avidin and the membrane protein BioY on cell surface. The reagent converts amines in the proximity of the binding pocket to azides and leaves the remaining amino groups in streptavidin untouched. Reagents of this novel class will find use in target identification as well as the selective functionalization and bioorthogonal protection of proteins.
Bioconjugate Chemistry published new progress about Avidins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, Category: esters-buliding-blocks.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics