Le Pogam, Pierre;Boustie, Joel;Richomme, Pascal;Denis, Antoine;Schinkovitz, Andreas published 《The inherent matrix properties of lichen metabolites in matrix-assisted laser desorption ionization time-of-flight mass spectrometry》 in 2017. The article was appeared in 《Rapid Communications in Mass Spectrometry》. They have made some progress in their research.Name: Methyl 2,4-dihydroxy-3,6-dimethylbenzoate The article mentions the following:
Light-absorbing secondary metabolites from lichens were recently reported to exhibit promising Laser Desorption Ionization (LDI) properties, enabling their direct detection from crude lichen extracts In addition, many of them display close structural homologies to com. Matrix-Assisted Laser Desorption Ionization (MALDI) matrixes, which is incentive for the evaluation of their matrical properties. The current study systematically evaluated the matrix effects of several structural classes of lichen metabolites: monoarom. compounds, quinone derivatives, dibenzofuran-related mols. and the shikimate-derived vulpinic acid. Their matrical properties were tested against a wide range of structurally diverse analytes including alkaloids, coumarins, flavonoids and peptides. Triplicate automatic pos.-ion mode MALDI analyses were carried out and ionization efficiencies were compared with those of structurally related reference matrixes (i.e. DHB, HCCA, dithranol and usnic acid) in terms of (1) analyte absolute intensities and (2) Matrix Suppressing Effect (MSE) scores. Monoarom. lichen metabolites revealed matrical properties similar to those of DHB when obtained under comparable exptl. conditions. Likewise, anthraquinone metabolites triggered ionization of tested analytes in a similar way to the structurally related dithranol. Finally, dibenzofuran derivatives displayed a broad ionization profile, reminiscent of that of (+)-usnic acid. Thus, lichen metabolites exhibit interesting MALDI matrix properties, especially for medium and low mol. weight analytes. For many of the tested mols., matrix ion formation was very limited. This proof-of-concept study paves the way for follow-up studies to assess the matrix properties of lichen metabolites against a wider array of analytes as well as adapting exptl. settings to individually optimize the performance of successfully tested candidates. The experimental procedure involved many compounds, such as Methyl 2,4-dihydroxy-3,6-dimethylbenzoate (cas: 4707-47-5) .
Methyl 2,4-dihydroxy-3,6-dimethylbenzoate(4707-47-5) is used in biological study as role of androgens in inducing distinct response of epithelial-mesenchymal transition factors in human prostate cancer cells.Name: Methyl 2,4-dihydroxy-3,6-dimethylbenzoate
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