Researchers who often do experiments know that organic synthesis is a process of preparing more complex target molecules from simple raw materials through one or more chemical reactions. Generally, it requires fewer steps, and cheap raw materials. 111-61-5, name is Ethyl stearate, A new synthetic method of this compound is introduced below., SDS of cas: 111-61-5
Example 5 Production of Octadecanedioic Acid (?ODDA?) by an Engineered Microorganism (0063) A one milliliter frozen glycerol stock of Candida sp. yeast strain sAA2178 (disclosed in international patent application PCT/US2013/076664) was inoculated into a 500 ml baffled flask containing about 80 ml of SP92 culture medium (described in Example 2 above) under sterile conditions, and the flask was capped with a foam plug and placed on a shaker at 30 C. and 250 rpms for about 24 hours. This culture was used to inoculate three 500 ml baffled flasks containing about 80 ml of SP92 culture medium at a starting optical density of 600 nm of 0.4. The flasks were incubated at 24 hours at about 30 C. and 250 rpm after which the three flasks were combined. This combined culture was divided into three aliquots which were used to inoculate three containing 2.5 L baffled flasks containing SP92 (75 g/L dextrose) and having initial optical density of 600 nm of 2; these flasks were incubated for about 24 hrs at about 30 C. and 250 rpms. (0064) These cultures were used to inoculate about 200 L of 1.5¡ÁKA media (described in Example 2) containing about 40.5 g/L of dextrose in a 300 L working volume stir tank fermenter. The fermenter was kept at about 34 degrees Celsius, agitation was kept at about 400 rpm, the airflow was about 1 VVM and the pH was set at about 5.8 using NaOH as base. Once dextrose was depleted the pH was increased to 6.0, glucose and ethyl-stearate mixture (74% ethy-stearate, 18% stearic acid, 8% methyl stearate) were each fed into the fermenter at about 1.35 g/L-h and 0.9 g/L-h respectively for the duration of the 100 hour fermentation, however from hour 40 through hour 48, the rate of ethyl stearate mixture was increased to 1.8 g/L hr. The resulting broth had a concentration of dodecanedioic acid of about 40 g/L as determined by GC analysis.
The basis of chemical reaction formula synthesis, the synthesis route is composed of some specific reactions and combined according to certain logical thinking. We look forward to the emergence of more reaction modes in the future.
Reference:
Patent; Verdezyne, Inc.; LAPLAZA, Jose; US2015/361024; (2015); A1;,
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics