Chemistry, like all the natural sciences, begins with the direct observation of nature¡ª in this case, of matter.4897-84-1, Name is Methyl 4-bromobutanoate, SMILES is O=C(OC)CCCBr, belongs to esters-buliding-blocks compound. In a document, author is Zhang, Guang-Xiang, introduce the new discover, Product Details of 4897-84-1.
Discovery and Engineering of a Novel Baeyer-Villiger Monooxygenase with High Normal Regioselectivity
Baeyer-Villiger monooxygenases (BVMOs) are remarkable biocatalysts for the Baeyer-Villiger oxidation of ketones to generate esters or lactones. The regioselectivity of BVMOs is essential for determining the ratio of the two regioisomeric products (normal and abnormal) when catalyzing asymmetric ketone substrates. Starting from a known normal-preferring BVMO sequence from Pseudomonas putida KT2440 (PpBVMO), a novel BVMO from Gordonia sihwensis (GsBVMO) with higher normal regioselectivity (up to 97/3) was identified. Furthermore, protein engineering increased the specificity constant (k(cat)/K-M) 8.9-fold to 484 s(-1) mM(-1) for 10-ketostearic acid derived from oleic acid. Consequently, by using the variant GsBVMO(C308L) as an efficient biocatalyst, 10-ketostearic acid was efficiently transformed into 9-(nonanoyloxy)nonanoic acid, with a space-time yield of 60.5 g L-1 d(-1). This study showed that the mutant with higher regioselectivity and catalytic efficiency could be applied to prepare medium-chain omega-hydroxy fatty acids through biotransformation of long-chain aliphatic keto acids derived from renewable plant oils.
Note that a catalyst decreases the activation energy for both the forward and the reverse reactions and hence accelerates both the forward and the reverse reactions. you can also check out more blogs about 4897-84-1. Product Details of 4897-84-1.