Chemistry is traditionally divided into organic and inorganic chemistry. The former is the study of compounds containing at least one carbon-hydrogen bonds. 120-61-6, Name is Dimethyl terephthalate, molecular formula is C10H10O4, belongs to esters-buliding-blocks compound, is a common compound. In a patnet, author is Lu, Fengniu, once mentioned the new application about 120-61-6, Recommanded Product: Dimethyl terephthalate.
Colorimetric alkaline phosphatase activity detection by integrating phosphorylation-mediated sulfydryl protection/deprotection and fluorosurfactant stabilized gold
Protection/deprotection of thiolates are widely used for the design of sensing systems by producing free sulfydryl (-SH) groups. However, most conventional protection/deprotection strategies reported so far suffer poor protection and/or difficult release of -SH group. In this study, phosphorylation/dephosphorylation strategy that exhibits efficient protection and facile liberation of -SH group via the formation of a phosphate-thiol ester, was utilized to develop colorimetric probe for alkaline phosphatase (ALP) activity evaluation. The proposed probe was composed of a cysteamine S-phosphate as a model thiolate substrate and Zonyl FSN-100 functionalized gold nanoparticles (FSN-AuNPs) as reporters. In the presence of ALP, the cysteamine S-phosphate hydrolyzed to generate cysteamine, which aggregated FSN-AuNPs with detectable spectroscopic changes through nanointerface interactions. The changes in UV-vis absorption spectroscopy allowed a detection limit of ALP at 0.1 unit/L. The specific enzyme catalytic hydrolysis provided high selectivity toward ALP over other proteins, small molecules, and metal ions. The practicability of the sensor was validated by accurate examination of ALP in blood samples. In addition, by integrating the conventional ELISA technique and the proposed phosphorylation tactic, sensitive immunoglobulin G detection was achieved.
We¡¯ll also look at important developments in the pharmaceutical industry because understanding organic chemistry is important in understanding health, medicine, 120-61-6. The above is the message from the blog manager. Recommanded Product: Dimethyl terephthalate.