Reference of 110661-91-1, Children learn through play, and they learn more than adults might expect. Science experiments are a great way to spark their curiosity, 110661-91-1, Name is tert-Butyl 4-bromobutanoate, SMILES is O=C(OC(C)(C)C)CCCBr, belongs to esters-buliding-blocks compound. In a article, author is Marshall, James R., introduce new discover of the category.
Screening and characterization of a diverse panel of metagenomic imine reductases for biocatalytic reductive amination
Finding faster and simpler ways to screen protein sequence space to enable the identification of new biocatalysts for asymmetric synthesis remains both a challenge and a rate-limiting step in enzyme discovery. Biocatalytic strategies for the synthesis of chiral amines are increasingly attractive and include enzymatic asymmetric reductive amination, which offers an efficient route to many of these high-value compounds. Here we report the discovery of over 300 new imine reductases and the production of a large (384 enzymes) and sequence-diverse panel of imine reductases available for screening. We also report the development of a facile high-throughput screen to interrogate their activity. Through this approach we identified imine reductase biocatalysts capable of accepting structurally demanding ketones and amines, which include the preparative synthesis of N-substituted beta-amino ester derivatives via a dynamic kinetic resolution process, with excellent yields and stereochemical purities. High-throughput biocatalytic screening and metagenomics have been used to discover over 300 imine reductases (IREDs) and subsequently produce a sequence-diverse panel of IREDs suitable for optimizing the synthesis of chiral amines. Additional characterization identified biocatalysts that accommodate structurally demanding amines and ketones for enzymatic reductive aminations.
Reference of 110661-91-1, Because enzymes can increase reaction rates by enormous factors and tend to be very specific, typically producing only a single product in quantitative yield, they are the focus of active research.you can also check out more blogs about 110661-91-1.