Chemistry is the experimental science by definition. We want to make observations to prove hypothesis. For this purpose, we perform experiments in the lab. , Formula: C8H14O2, 3681-71-8, Name is cis-3-Hexenyl acetate, molecular formula is C8H14O2, belongs to esters-buliding-blocks compound. In a document, author is Fernando, Wasundara, introduce the new discover.
Metabolism and pharmacokinetics of a novel polyphenol fatty acid ester phloridzin docosahexaenoate in Balb/c female mice
Flavonoids are known to undergo phase II metabolism and produce metabolites with similar or stronger biological effects compared to the parent flavonoids. However, the limited cellular uptake and bioavailability restrict their clinical use. We synthesized phloridzin docosahexaenoate (PZ-DHA), a novel fatty acid ester of polyphenol, through an acylation reaction with the aim of increasing the cellular availability and stability of the parent biomolecules, phloridzin (PZ) and docosahexaenoic acid (DHA). Here, we report metabolites and pharmacokinetic parameters of PZ-DHA, determined using ultra-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. PZ-DHA was taken-up by human (MDA-MB-231, MDA-MB-468, and MCF-7) and mouse (4T1) mammary carcinoma and human non-malignant mammary epithelial cells (MCF-10A) in cellular uptake assays. Our results suggested that the acylation improves the cellular uptake of PZ and stability of DHA within cells. In mouse hepatic microsomal assays, two major glucuronides of PZ-DHA, PZ-DHA-4-O-glucuronide and PZ-DHA-4 ‘-O-glucuronide (MW=923.02 g/mol), were detected. One tri-methylated- (4,4 ‘,6 ‘-O-trimethyl-PZ-DHA) (MW=788.88 g/mol) and one di-sulphated- (PZ-DHA-4,4 ‘-O-disulphide) PZ-DHA metabolite (MW=906.20 g/mol) were also identified. Intraperitoneal injections of PZ-DHA (100 mg/kg) into Balb/c female mice was rapidly absorbed with a serum C-max and T-max of 23.7 mu M and 60 min, respectively, and rapidly eliminated (t(1/2)=28.7 min). PZ-DHA and its metabolites are readily distributed throughout the body (V-d=57 mL) into many organs. We identified in vitro and in vivo metabolites of PZ-DHA, which could be tested for potential use to treat diseases such as cancer in multiple organ systems.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 3681-71-8. Formula: C8H14O2.