Chemistry is the science of change. But why do chemical reactions take place? Why do chemicals react with each other? The answer is in thermodynamics and kinetics, Recommanded Product: Methyl 4-methoxybenzoate, 121-98-2, Name is Methyl 4-methoxybenzoate, SMILES is C1=CC(=CC=C1OC)C(OC)=O, belongs to esters-buliding-blocks compound. In a document, author is Yoo, Wanki, introduce the new discover.
Identification, characterization, and immobilization of a novel YbfF esterase from Halomonas elongata
The YbfF esterase family, which has a bifurcated binding pocket for diverse ligands, could serve as excellent biocatalysts in industrial and biotechnological applications. Here, the identification, characterization, and immobilization of a novel YbfF esterase (YbfF(Halomonas elongata)) from Halomonas elongata DSM2581 is reported. Biochemical characterization of YbfF was carried out using activity staining, chromatographic analysis, kinetic analysis, activity assay, acetic acid release, and pH-indicator-based hydrolysis. YbfF(H. elongata) displayed broad substrate specificity, including that for p-nitrophenyl esters, glucose pentaacetate, tert-butyl acetate, and beta-lactam-containing compounds, with high efficiency. Based on a homology model of YbfF(H.elongata), Trp237 in the substrate-binding pocket, a critical residue for catalytic activity and substrate specificity was identified and characterized. Furthermore, crosslinked enzyme aggregates and nanoflower formation were explored to enhance the chemical stability and recyclability of YbfF(H.elongata). The present study is the first report of a YbfF esterase from extremophiles, and explains its protein stability, catalytic activity, substrate specificities and diversities, kinetics, functional residues, amyloid formation, and immobilization. (C) 2020 Elsevier B.V. All rights reserved.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 121-98-2. Recommanded Product: Methyl 4-methoxybenzoate.